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KMID : 1007519970060010026
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Food Science and Biotechnology
1997 Volume.6 No. 1 p.26 ~ p.29
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Comparison of Molecular Weight Distribution of Commercially Purified Oat and Barley β - glucans
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Lim Hye-Sook S
Pamela J White
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Abstract
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Commercial oat and barley β-glucans were fractionated by gel permeation chromatography (GPC). Total β-glucan contents (1-3), (1-4)-β-D-glucopyranosyl of each fraction, measured by flow injection analysis (FIA) and by the anthrone-sulfuric acid (ASA) method for total carbohydrates, were compared, and molecular weight (MW) distributions of oat and barley β-glucans were observed. Barley β-glucan showed two defined MW distributions of 9.8×10^7 to 3.4×10^7 g/mole and 2.7×10^5 to 2.7×10³g/mole. The observed MW range for oat β-glucan was about 4.0×10^6 to 2.0×10⁴g/mole. At a β-glucan concentration of below 0.02 ㎎/㎖, the FIA was not as sensitive as the ASA method.
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